Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MOPS 99.5 (titration), 1132-61-2, MFCD00006183
MOPS 99.5 (titration)
Alkali Scientific CellPro™ PBS Buffer, with Ca + Mg, 1X, pH 7.4, 500mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Medchemexpress LLC 20 mM HEPES, 150 mM NaCl, 240 mM Sucrose, pH 7.2 buffer | 10 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, meticulously adjusted to a pH of 7.2. It is specifically designed for the resuspension of human erythrocyte ghost preparations.
- Physiological buffer
- Composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose
- Adjusted to pH 7.2
- Used for resuspending human erythrocyte ghost preparations
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 500g
Linear Formula NH2C(CH2OH)3, ≥99.9% (titration), Molecular Weight 121.14, bp 219-220 °C/10 mmHg(lit.), mp 167-172 °C (lit.), Primary Standard and Buffer, crystalline, Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
AdipoGen PBS Endotoxin-free (sterile) (100 ml)
PBS Endotoxin-free (sterile) (100 ml), AdipoGen Life Sciences. Chemical. Endotoxin free buffer used for the dilution of TLRpure LPS and solubilisation of endotoxin-free reagents (e.g. ODNs).
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, MFCD00132476, 10pk
pH 8.0, powder, Synonym: TBS. Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); pH 8.0, at 25 °C.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 6.5) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Sigma Aldrich Fine Chemicals Biosciences DIPSO BioXtra pH 4.0 5.5 2100G
DIPSO BioXtra pH 4.0 5.5 2100G
FUJIFILM Biosciences PBS 1X-Dulbecco's Phosphate Buffered Saline Solution - Liquid
PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
Sigma Aldrich Fine Chemicals Biosciences BES sodium salt 99 titrati500G
BES sodium salt 99 titrati500G
Sigma Aldrich Fine Chemicals Biosciences BIS TRIS propane BioXtra 9250G
BIS TRIS propane BioXtra 9250G
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 9.5) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB