Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific ADA, [N (Carbamoylmethyl) iminodiacetic acid), 25 G
ADA (N-(Carbamoylmethyl) iminodiacetic acid) is a biochemical reagent available in a 25g bottle. This buffering agent is ideal for laboratory applications requiring precise pH regulation. ADA is commonly used in chromatography, protein studies, and enzyme assays, providing a stable pH environment during various biochemical experiments. Its ability to maintain a controlled pH is essential for accurate results in protein purification, cell culture, and molecular biology studies. The 25g size is perfect for smaller-scale experiments or routine laboratory use where reliable buffering capacity is necessary for optimal results.
Sigma Aldrich Fine Chemicals Biosciences MOPS BioUltra, for molecular biology, >=99.5% (T) | 1132-61-2 | MFCD00006183 | 500G
MOPS BioUltra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 500G
Crescent Chemical Co Inc MORPHOLINOPROPANE SULFONICACID
Morpholinopropane sulfonic acid analytical grade pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies. Size - 100G Sto
National Diagnostics MOPS-SDS Running Buffer (20X), 1L
Reliable and Economical, 18 Megohm Water, and 0.2 Micron Filtered. Ideal running buffer for high resolution SDS PAGE of proteins. 20X concentrate makes a working solution of 50mM Tris MOPS, 0.1% SDS and 1mM EDTA.
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 250 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255317.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 250 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurine ACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agent inbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+. Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 7.0
Biotang Inc HEPES Protein Extraction Buffer (5X), 500 ml
HEPES Protein Extraction Buffer (5X), 500 ml.
Bioworld ACE MES Buffer, 50mM, pH 6.0, 100 mL
Product DescriptionA.C.E.™ MES (compound 2-(N-morpholino)ethanesulfonic acid) buffer has a concentration of 50 mM with a pH level of 6.0. The buffer is biotechnology grade and is formulated for capillary electrophoresis. Buffer is made with 3D water and filtered through 0.22 µ filter. Product is shipped at room temperature.
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt >=99% (titration) | 76836-02-7 | MFCD00064349 | 500G
PIPES disodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 500G
Miltenyi Biotec, Inc. MACSima Running Buffer 1.5 L
MACSima™ Running Buffer to use in the MACSima Imaging System.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 100g
CAPS | Purity: 99% | MW: 221.32 | 1135-40-6 | MFCD00003837 | 100g
![Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660483.jpg-250.jpg)
Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 100 G
TAPS, also known as Tris(hydroxymethyl) aminopropanesulfonic acid, is a high-purity buffering agent widely used in laboratory applications. The free acid form of TAPS is essential for preparing stable and reliable buffers, especially in experiments that require tight control over pH. It is commonly used in biochemical, molecular biology, and clinical research applications. The 100g quantity of TAPS is suitable for preparing a variety of buffer solutions for electrophoresis, enzyme assays, and other analytical procedures. The high purity ensures that the reagent will not interfere with sensitive analytical reactions, making it a go-to choice for researchers seeking consistency and precision in their experiments.